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KMID : 0869420180160010025
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Journal of Jeahan Oriental Medical Academy
2018 Volume.16 No. 1 p.25 ~ p.36
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Anti-inflammatory Effect of Arctium lappa L. Root Ethanol Extract in RAW264.7 Cells
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Seo Ho-Seok
Park Chung-A
Jee Seon-Young
Hwangbo Min
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Abstract
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Objectives：Root of Arctium lappa L. has been used in Korean Traditional Medicine to treat odontalgia, throat pain and dizziness. In the present study, we investigated effect of A. lappa root ethanol extract (ALR_E) on lipopolysaccharide (LPS)- induced acute inflammation in RAW264.7 cells, a murine macrophage cell line.
Methods：After root and seed of A. lappa were extracted by using water and ethanol, RAW264.7 cells were pretreated with 30-1000 μg/mL of each extract and then exposed to 1 μg/mL of LPS for 6 or 18 h. Cell viability was determined by MTT assay. Levels of nitric oxide (NO), prostaglandin E2, tumor necrosis factor-α, interleukin-1β, interleukin-6, monocyte chemoattractant protein-1 were measured in the medium. mRNA levels of inducible nitric oxide synthase, cyclooxygenase-2, and pro-inflammatory cytokines genes were determined by real-time PCR analyses.
Results：Pretreatment with ethanol extract of seed or root of A. lappa significantly inhibited LPS-stimulated NO production without any cytotoxicity. In addition, ALR_E decreased the mRNA transcription of inducible nitric oxide synthase and cyclooxygenase-2 by LPS. Moreover, ALR_E attenuated LPS- mediated induction of tumor necrosis factor-α, interleukin-1β, interleukin-6, and monocyte chemoattractant protein-1 in RAW264.7 cells.
Conclusions：These results suggest that ALR_E has an ability to prevent inflammation in macrophages via inhibiting NO, prostaglandin E2, and proinflammatory cytokines production.
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KEYWORD
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inflammation, Root of Arctium lappa L., Lipopolysaccharide (LPS), RAW264.7 cell
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